Journal: NPJ Science of Food

Article Title: The Artemisia argyi oil reduces high-fat diet-induced obesity by enhancing thermogenesis in brown adipose tissue

doi: 10.1038/s41538-025-00633-2

Figure Lengend Snippet: A Western blot analysis showing the expression of UCP1, PGC1α, PPARα, PRDM16, and AGT in the BAT of mice from the NCD, HFD, LA, MA, and HA groups. B – F The mRNA levels of Ucp1 , Pgc1α , Pparα , Prdm16 , and Agt in the BAT of mice (n = 6). G Immunofluorescence (IF) analysis for the UCP1 expression in BAT of mice. Scale bar, 50 μm. H Protein expression levels of COXII and COXIV in BAT of mice. The mRNA levels of Cox8b ( I ) and Cox5b ( J ) in BAT were determined by qPCR analysis. * denote the level of statistical significance. ns no significance, * p < 0.05, ** p < 0.01, *** p < 0.001. Data are presented as mean ± SEM. Statistical analyses were performed using one-way ANOVA followed by Tukey’s test ( B – F , I – J ).

Article Snippet: Membranes were blocked with Fastest Blocking Reagent (HYCEZMBIO, Cat#HYC00811) and incubated with primary antibodies at the following dilutions: UCP1 (Diagbio, Cat#db9840) (dilution 1:1000), PRDM16 (Santa, Cat#sc-517625) (dilution 1:1000), PGC1α (SAB, Cat#37818) (dilution 1:1000), β-actin (Sino Biological, Cat#109444-T36) (dilution 1:20000), FASN (BOSTER, Cat#PB9865) (dilution 1:1000), PPARα (Biodragon, Cat#BD-PB4080) (dilution 1:1000), HSL (ZENBIO, Cat#344379) (dilution 1:1000), Flag (Sigma-Aldrich, Cat#F7425) (dilution 1:1000), HA (Bioss, Cat#BMS0966M) (dilution 1:1000), PPARγ (Bioworld, Cat#BS79617) (dilution 1:1000), ATGL (Abways, Cat#CY8408) (dilution 1:1000), ACC1 (HABIO, Cat#ET1609-77) (dilution 1:1000), IL10 (Solarbio, Cat#K009382P) (dilution 1:1000), TNFα (ELK Biotechnology, Cat#EA251) (dilution 1:1000), ZFP516 (GenScript) (dilution 1:1000), and LSD1 (PTM BIO, Cat#PTM-5960) (dilution 1:1000).

Techniques: Western Blot, Expressing, Immunofluorescence

Journal: NPJ Science of Food

Article Title: The Artemisia argyi oil reduces high-fat diet-induced obesity by enhancing thermogenesis in brown adipose tissue

doi: 10.1038/s41538-025-00633-2

Figure Lengend Snippet: A The diagram illustrates that the BAT SVFs were isolated and differentiated into mature brown adipocytes, followed by AAO treatment for western blot analysis, qPCR, and OCR analysis. B The TG content in the cells of each group. C The protein expression levels of UCP1, PRDM16, AGT, and PPARγ in the cells of each group. D – G The mRNA expression levels of Ucp1 , Prdm16 , Agt , and Pparγ . H – J BAT SVF cells were differentiated into brown adipocytes, followed by treatment with AAO. Oligomycin, FCCP, and Rotenone/Antimycin were added at the specified time points, indicated by the arrows, and the OCR data are shown in ( H ). The average basal and maximal respiration rates are presented in ( I ) and ( J ). * denote the level of statistical significance. ns, no significance, * p < 0.05, ** p < 0.01, *** p < 0.001. Data are presented as mean ± SEM. Statistical analyses were performed using one-way ANOVA followed by Tukey’s test ( B , D – G , I , J ).

Article Snippet: Membranes were blocked with Fastest Blocking Reagent (HYCEZMBIO, Cat#HYC00811) and incubated with primary antibodies at the following dilutions: UCP1 (Diagbio, Cat#db9840) (dilution 1:1000), PRDM16 (Santa, Cat#sc-517625) (dilution 1:1000), PGC1α (SAB, Cat#37818) (dilution 1:1000), β-actin (Sino Biological, Cat#109444-T36) (dilution 1:20000), FASN (BOSTER, Cat#PB9865) (dilution 1:1000), PPARα (Biodragon, Cat#BD-PB4080) (dilution 1:1000), HSL (ZENBIO, Cat#344379) (dilution 1:1000), Flag (Sigma-Aldrich, Cat#F7425) (dilution 1:1000), HA (Bioss, Cat#BMS0966M) (dilution 1:1000), PPARγ (Bioworld, Cat#BS79617) (dilution 1:1000), ATGL (Abways, Cat#CY8408) (dilution 1:1000), ACC1 (HABIO, Cat#ET1609-77) (dilution 1:1000), IL10 (Solarbio, Cat#K009382P) (dilution 1:1000), TNFα (ELK Biotechnology, Cat#EA251) (dilution 1:1000), ZFP516 (GenScript) (dilution 1:1000), and LSD1 (PTM BIO, Cat#PTM-5960) (dilution 1:1000).

Techniques: Isolation, Western Blot, Expressing